椎间盘退变的形态学分型中转化生长因子-β1表达的临床意义

摘要

目的 探讨退变椎间盘分型中转化生长因子(TGF-β1)表达的意义.方法 收集正常与退变椎间盘组织,并根据病理改变把退变椎间盘组织分为Ⅳ级,采用HE染色,Western bloting和RT-PCR方法分析TGF-β1的表达情况.结果 HE染色、Westernbloting和RT-PCR均显示:在正常组织中TGF-β1有表达；在病变组织中并随病理分型增加TGF-β1随之增加,退变与正常组比较以及IV型与I型比较具有及显著差异(P＜0.01).结论 TGF-β1是引起椎间盘退变的重要原因,并且与病理的形态学分型密切相关.%Objective: To explore the role of transforming growth factor-β1 (TGF-βl) in intervertebral disc degeneration and its association with the pathological grading of disc degeneration.Methods Normal and degenerative intervertebral disc tissues were collected were classified into 5 grades of increasing degenerative changes.HE staining,immunohistochemistry,TUNEL staining and RT-PCR were used to detect the expression of TGF-βl in the disc tissues.Results Immunohistochemistry and RT-PCR showed positive expressions of TGF-β1 and Bcl-2 in normal disc tissues,where Bax was expressed at have a trace level.In the degenerative disc tissues,TGF-βl expression increased with the pathological grades;the expression levels of TGF-p 1 showed significant differences between degenerative and normal tissues and between grade IV and grade I disc tissues (P< 0.01).Conclusion TGF-βl is an important factor participating in the disc degeneration and its expression level is closely related to the pathological grade of degenerative discs.