牛蒡子苷对晚期氧化蛋白产物诱导小鼠足细胞转分化的影响

摘要

目的 探讨牛蒡子苷对晚期氧化蛋白产物(AOPP)诱导小鼠足细胞转分化的影响.方法 用200 μg/ml AOPP刺激小鼠足细胞24h,同时加入牛蒡子苷(浓度分别为50、100、200、400 μmol/L)进行干预,采用Western blotting检测内质网应激标志性蛋白Grp78、CHOP及平滑肌激动蛋白(α-SMA)表达水平.结果 牛蒡子苷干预后可抑制小鼠足细胞Grp78、CHOP、α-SMA蛋白表达水平,且在一定范围内呈剂量依赖性.结论 AOPP可通过引起小鼠足细胞内质网应激(ERS)从而导致上皮-间充质转分化(EMT),而牛蒡子苷则可通过减轻ERS从而逆转EMT,为治疗肾脏纤维化提供新的研究方向.%Objective To study the effect of arctiin on mouse podocyte epithelial-mesenchymal transition (EMT) induced by advanced oxidation protein products (AOPP). Methods Mouse podocytes were stimulated by 200 ug/ml AOPP for 24 h in the presence of 50, 100, 200, and 400 μmol/L arctiin. The expressions of o-smooth muscle actin, Grp78 and CHOP were detected using Western blotting. Result The expressions of a-SMA, Grp78 and CHOP were inhibited by arctiin, showing a dose-dependent effect within a given range of arctiin concentration. Conclusion AOPP causes endoplasmic reticulum stress to induce EMT of mouse podocytes, and arctiin can decrease EMT by alleviating the stress. This finding sheds light on a new scope of research of renal fibrosis.