Na+、K+、葡萄糖及甘油对高体雅罗鱼精子活力的影响

摘要

[目的]明确水体介质参数与高体雅罗鱼精子活力的关系,解决高体雅罗鱼人工繁殖受精率低及鱼苗鱼种生产效率低的技术难题.[方法]以快速运动时间(FT)、寿命时间(LT)和精子激活率为评价指标,探究Na+、K+、葡萄糖及甘油对高体雅罗鱼精子活力的影响.[结果]Na+浓度为135 mmol/L时高体雅罗鱼精子活力最强,FT为71 s,LT为1020 s,精子激活率为95%;K+浓度为5 mmol/L时高体雅罗鱼精子活力最强,FT为52 s,LT为280 s,精子激活率为80%;葡萄糖浓度为180 mmol/L时高体雅罗鱼精子活力最强,FT为78 s,LT为1200 s,精子激活率为95%;甘油浓度为180 mmol/L时高体雅罗鱼精子活力最强,FT为79 s,LT为1380 s,精子激活率为90%.[结论]适宜浓度的Na+、K+、葡萄糖及甘油具有单独提高高体雅罗鱼精子活力的作用,但高浓度Na+、K+、葡萄糖及甘油对高体雅罗鱼精子的激活率呈明显抑制作用.实际生产中,可选择135 mmol/L Na+、5 mmol/L K+、180 mmol/L葡萄糖或180 mmol/L甘油作为高体雅罗鱼精子的激活液,提高其人工繁殖生产效率.%[Objective]The relationship between water medium parameters and sperm motility of Leuciscus idus was studied to solve the technical problems of low fertility rate and and low production efficiency of fry in L. idus artificial breeding. [Method]With fast moving time(FT), life time(LT) and sperm activation rate as evaluation indexes, the effects of Na+, K+, glucose and glycerinon on sperm motility of L. idus were studied. [Result]When the concentration of Na+was 135 mmol/L, the sperm motility of L. idus was the strongest, with FT being 71 s, LT 1020 s, and sperm activation rate 95%. When the concentration of K+ was 5 mmol/L, the sperm motility of L. idus was the strongest, with FT being 52 s, LT 280 s , and sperm activation rate 80%. When the concentration of glucose was 180 mmol/L, the sperm motility of L. idus was the strongest, with FT being 78 s, LT 1200 s, and sperm activation rate 95%. When the concentration of glycerinon was 180 mmol/L, the sperm motility of L. idus was the strongest, with FT being 79 s, LT 1380 s, and sperm activation rate 90%. [Conclusion]Appropriate concentrations of Na+,K+,glucose and glycerol alone can increase L. idus sperm motility. But high concentrations of Na+, K+, glucose and glycerol inhibit sperm activation rate. In actual cultivation, 135 mmol/L Na+, and 5 mmol/L K+, 180 mmol/L glucose or 180 mmol/L glycerol can be chosen as sperm activation medium for L. idus to im-prove the efficiency of artificial breeding.