分子印迹-流动注射分子荧光法测定水中双酚A的研究

摘要

Using Bisphenol A ( BPA ) as the template,acrylamide as monomers,Pentaerythritol Triacrylate PETA as crosslinking agent,we synthesized MIPM in aquosity system with suspension polymerization,on which surface there are some holes.The absor-bance of MIPM to hydroquinone will no longer increased after 110 minutes,it means that it reaches the saturation which were 34.38 μmol · L-1.The separate genes of MIPM was 22.88 with phenol as competitiors; The MIPM had the better effect of adsorption and selectivity.Using BPA - MIPM as molecule recognizates,Based on the enhancing effect of β - cyclodextrin on the flu-orescence from BPA in acidic solution,we set up a new method which was molecular imprinted technique - flow injection - che-moluminescence analysis to detect hydroquinone.The result shows that the range of hydroquinone concentration from 0.1 βg · L-1 to 100 βg · L-1 with adsorbed by MIPM is linearly dependent,which relativity coefficient is 0.9993and the relative standard deviation is 0.79% (20 βg · L-1 ,n = 11) ,the detection limit is 0.08 (βg · L-1.Using the method to detecting hydroquinone from the water sample,the recoveries of hydroquinone is 98.6% - 103.9% ,the result is pretty good.%以双酚A(BPA)为模板分子,以丙烯酰胺为功能单体,季戊四醇三丙烯酸酯为交联剂,采用悬浮聚合法,在水性体系中制备了表面带孔的分子印迹聚合微球( MIPM),制备的BPA分子印迹聚合微球( BPA - MIPM)吸附平衡时间为110 min,饱和吸附量为34.38 μmol·g-1.BPA - MIPM以苯酚为竞争分子,分离因子达到22.88；制备的MIPM具有良好的选择吸附性能和良好的再生性能.以BPA - MIPM为分子识别物,基于在pH为1的酸性介质中,β-环糊精对双酚A荧光强度的增强作用,建立测定BPA的分子印迹-流动注射分子荧光法.测得BPA在0.1～100 μg·L-1线性范围内具有很好的相关性,相关系数r为0.9993,相对标准偏差(20μg·L-,n=11)为0.79％,检出限为0.08 μg·L-,用该方法测水中BPA的加标回收率为98.6％ ～ 103.9％,结果较好.