Objective To examine the effects of insulin on cell proliferation, nitric oxide (NO) release and nitric oxide synthase (NOS) gene expression in bovine aortic endothelial cells ( BAEC ) . Methods The mi togenesis was assessed by MTT method; the products of NO in the culture media, by Griess reaction; and the levels of NOS mRNA in BAEC , by RT/PCR technique. Results BAEC were not responsive to the growth-promoting effects of insulin. Stimulation with insulin resulted a dose-dependent rise of NO in the culture supernatants 2h later, with a maximum at 12～24h and a decline at 24h. This rise was inhibited by an inhibitor of NOS (L-NAME). NOS mRNA increased slightly in BAEC without statistical significance. Conelusion The study suggested that the insulin-induced NO release might be caused directly by NOS activation.
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机译：目的探讨胰岛素对细胞增殖，一氧化氮（NO）释放和一氧化氮的影响牛主动脉内皮细胞（BAEC）中的合成酶（NOS）基因表达。方法通过MTT评估MI生物功能方法;通过Griess反应的培养基中的否则的产品;和RT / PCR技术的BAEC中NOS mRNA水平nique。结果BAEC对胰岛素的生长促进作用并无敏感。胰岛素的刺激导致了一个在培养上清液中的剂量依赖性升高2h以后，最大12〜24h，24小时下降。这幅度是由NOS（L-NAME）的抑制剂抑制。 NOS mRNA在BAEC略微增加而不统计显着性。 Conelu.该研究表明，胰岛素诱导的没有释放可能是直接通过NOS激活引起的。
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