不明原因复发性流产患者抑制型杀伤细胞免疫球蛋白样受体基因表达频率

摘要

Objective; To investigate the association of inhibitory killer cell immunoglobulin-like receptor (KIR) gene in women with unexplained recurrent spontaneous abortion(URSA).Methods: The expressions of inhibitory killer cell immunoglobin-like receptor genes in 48 women with unexplained recurrent spontaneous abortion and 50 health women as control were detected by PCR-sequence specific primer method.Results: The frequency of inhibitory KIR2DL2 in the URSA group (52. 1%) significantly increased than the control group(26. 0%) (P<0. 05). The percentage of patients possessing of 3 inhibitory KIR genes in the URSA group(50. 0%)was significantly higher than the control group(24. 0%)(F<0. 05).Conclusions: The increased frequency of inhibitory KIR2DL2 might influence the susceptibility to URSA. Imbalance of inhibitory and activation KIR could contribute to the URSA.%目的 探讨抑制型杀伤细胞免疫球蛋白样受体(KIR)基因在不明原因复发性流产(URSA)发病机制的作用.方法 采用序列特异性引物聚合酶链反应(PCR-SSP)方法检测48例URSA患者与50例对照组女性抑制型KIR基因的表达.结果 URSA患者KIR2DL2基因频率(52.1％)增高,与对照组(26.0％)比较有显著差异(P＜0.05)；URSA患者拥有3个抑制型KIR基因的阳性率增高(50.0％),与对照组(24.0％)比较有显著差异(P＜0.05).结论 KIR2DL2基因频率增高可能是URSA发病的易感因素,子宫自然杀伤(uNK)细胞表面抑制型与活化型KIR受体表达失衡可能是URSA发病机制之一.