The toxicity of organophosphorus, carbamate, neonicotinoid, pyrethroid insecticides to Drosophila melanogaster were determined using the method of feeding on toxic medium. The result showed that the tested D. Melanogasterwere very sensitive to pyrethroid and organophosphorus insecticides. After 3h treatment, the LC50 were less than or near to maximum residue limit ( MRL). However, the D. Melanogaster was less sensitive to carbamate and neonicotinoid insecticides. The sensibility of D. Melanogaster to pyrethroid and organophosphorus pesticides mixed in the homogenate of vegetable were future determined, and the same result was obtained. After 3h treatment, the LC50 of DDVP, Chlorpyrifos and Cyfluthrin were 0. 123mg/kg, 0. 912mg/kg and 0. 021mg/kg respectively , which were lower than the MRL ( GB ). This experiment indicated that the tested insect D. Melanogaster should be feasible to be used as an indicator to detect pyrethroid and organophosphorus insecticides residues rapidly in vegetables.%采用培养基混毒法,测定了黑腹果蝇(Drosophila melanogaster)对有机磷、氨基甲酸酯、新烟碱类和拟除虫菊酯类杀虫剂的敏感性.结果显示,所试黑腹果蝇对拟除虫菊酯类、有机磷类杀虫剂非常敏感,3h内LC50低于或接近于最大残留限量(MRL)；对氨基甲酸酯类杀虫剂敏感性稍差,对新烟碱类杀虫剂不敏感.进一步测定黑腹果蝇对蔬菜匀浆中有机磷类和拟除虫菊酯类杀虫剂的敏感性,结果与培养基混毒法一致,黑腹果蝇保持了对这两类杀虫剂的高度敏感性,3h内敌敌畏、毒死蜱和氯氟氰菊酯的LC50分别是0.123mg/kg、0.912mg/kg和0.021 mg/kg,均低于中国国家标准的最大残留限量.所试黑腹果蝇可以作为指示昆虫用于蔬菜中有机磷类和拟除虫菊酯类杀虫剂残留的快速检测.
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