4种合成活性肽对小鼠生长性能和免疫机能的影响

摘要

A experiment was conducted to discuss the growth performance, immune organ index and immune function of four synthetic bioactive peptides (peptide 1, peptide 2, peptide 3 and peptide 4 were consist of 15, 7, 9 and 7 amino acids respectively, the linear peptides compound by solid-phase synthesis manual) on mice by gastric perfusion.90 clean health mice were randomly divided into 9 groups with 10 mice per group with, the test groups were administered with 10 μg/kg and 50 μg/kg body weight of four synthetic bioactive peptides, the blank control group was fed with equivalent distilled water.The test period was 32 days.The result showed that, (1) There was no significantly difference on the weight and the average daily feed consumption of the mice feeding synthetic bioactive peptides 15 days and 32 days among the experiment groups and control group(P>0.05).(2) Bioactive peptide 3 feeding 15 days 50 μg/kg body weight can significantly improve serum IgG and γ-interferon content on mice (P0.05).Cardiac, spleen and kidney coefficients were no significant different in the each group (P>0.05).On the mice feeding synthetic bioactive peptides 32 days, bioactive peptide 2,3 and 4 feeding 50 μg/kg body weight could significantly improve its heart, liver and kidney coefficient (P0.05).(2)小鼠灌喂活性肽15 d,50 μg/kg体重肽3能显著提高小鼠血清IgG和IFN-γ含量(P0.05);心脏、脾脏和肾脏系数各组之间差异均不显著(P>0.05).小鼠灌喂活性肽32 d时,50 μg/kg体重活性肽2、肽3、肽4对小鼠心脏、肝脏和肾脏系数有显著提高作用.综上可见,4种人工合成生物活性肽对小鼠生长性能未见明显影响,对免疫机能和脏器系数有一定的促进作用.