Objective: To study the biological characteristics and osteogenic potency of adipose-derived stem cells(ASCs). Methods : Cells were isolated from the bilateral inguinal fat pads by collagenase 1 digestion and cultured. Cell morphology was observed under the phase contrast microscope. The cell proliferation was tested by MTT assay. The surface markers of the cells were analyzed by flow cytometry. Passage 3 cells were cultured in osteogenic and adipogenic inducing medium for 7, 28 and 21 days respectively, and verified with ALP staining, Alizarin red staining and Oil Red 0 staining respectively. Results; The cells presented fibroblast-like or polygon shape. The growth curve was like " S" shape reflecting strong proliferative ability. ASCs expressed CD29 and CD90, but not CD34 and CD45. The ALP staining, Alizarin red staining and Oil Red 0 staining showed positive reaction. Conclusion: ASCs can be cultured from SD rat fat tissue and can be induced to osteogenic differentiation.%目的:观察SD大鼠脂肪干细胞(adipose-derived stem cells,ASCs)体外培养的基本生物学特性、鉴定其诱导成骨分化潜能.方法:无菌条件下取6周龄SD大鼠腹股沟处脂肪组织,0.1％Ⅰ型胶原酶消化,分离培养.显微镜下观察细胞形态；采用MTT比色法绘制细胞生长曲线；用流式细胞仪检测细胞表面标志物；取第3代细胞行成骨和成脂诱导培养,分别用碱性磷酸酶(ALP)、茜素红染色和油红O染色鉴定成骨诱导效果.结果:从SD大鼠脂肪组织中分离获得的ASCs呈长梭形或多角形,呈集落生长；细胞生长曲线呈“S”型,具有较强的增殖能力；流式细胞仪分析示:CD29、CD90高表达,CD34、CD45低表达；成骨诱导后ALP染色及茜素红染色阳性,成脂诱导后油红O染色阳性.结论:分离培养的细胞为ASCs,具有成骨潜能.
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