Objective: To study the pathological significance of mast cell degranulation in human periodontitis. Methods: A total of SO donors including 17 with mild chronic periodontitis, 18 with advanced chronic periodontitis (n = 18) and 15 of healthy subjects were involved in this study. Gingiva specimens were fixed in 10% buffered formalin, stained with hematoxylin and eosin for his-topalhologic observation, stained with toluidine blue for identification of mast cells and its degranulation, and stained with picric-sinus red for detecting fibrosis degree in periodontal tissues. Results: More mast cells with more degranulation were observed in advanced chronic periodontitis groups lhan in the other 2 groups(P<0.01) and in mild periodontitis group than in healthy control group(P < 0.01). More severe pathogenesis with stronger fibrosis was found in advanced chronic periodontitis group than in mild chronic periodontitis group (P<0.05). Conclusion; Mast cells and their degranulation may contribute to the progression of periodontal disease and severity of tissue fibrosis.%目的:探讨肥大细胞脱颗粒在牙周炎病理中的意义.方法:将自愿接受研究的受试者分为3组:轻度慢性牙周炎组(轻度组,17例)、重度慢性牙周炎组(重度组,18例)、正常对照组(15例).取牙龈活检组织制作连续切片,HE染色在光镜下观察各组牙周组织的组织学改变；甲苯胺蓝染色法观察各组肥大细胞的数量及肥大细胞脱颗粒状况；天狼猩红染色法观察各组牙周组织纤维化的程度.结果:与正常对照组相比,各慢性牙周炎组的牙周组织中肥大细胞显著增多(p＜0.01)；重度组与轻度组相比较肥大细胞数量和脱颗粒率均明显升高(p＜0.01),牙周组织纤维化程度更加严重(p＜0.05).结论:肥大细胞的募集、脱颗粒和纤维化程度与牙周炎的严重程度相关,提示肥大细胞可能在牙周炎的发病和疾病进程中起着重要的作用.
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