HIF-1α对口腔癌裸鼠移植瘤生长及其CEACAM1及VEGF-C表达的影响

摘要

Objective:To explore the effects of HIF-1α on the growth of transplanted oral cancer and on the expression of CEACAM1 and VEGF-C in the tumor.Methods:Nude mouse model of oral cancer was established by transplantation of Tca8113 cells respectively treated by HIF-1α siRNA and negative control siRNA subcutaneously into right axillary region of nude mice.3 weeks after transplantation the mice were sacrificed,the tumor volum and weight were measured.The tumor tissue was examined by ELISA method for the detection HIF-1α protein expression,by real-time quantitative PCR and western blot for the detection of mRNA and protein expression of HIF-1α,CEACAM1 and VEGF-C respectively.Results:The volume and weight of the transplanted tumor in HIF-1 α siRNA group were significantly less than those in the control group(P＜0.05),CEACAM1 and VEGF-C mRNA and protein were down-regulate in HIF-1α siRNA group (P＜0.05).Conclusion:HIF-1α expression is positively related to the expression of CEACAM1 and VEGF-C in the regulation of oral tumor growth.%目的:建立口腔癌裸鼠移植瘤模型,探究HIF-1α对CEACAM1及VEGF-C表达的影响机制.方法:分别将Tca8113细胞、siRNA阴性对照Tca8113细胞、HIF-1α干扰的Tca8113细胞接种到裸鼠的右腋部皮下,建立口腔癌裸鼠移植瘤模型.观察各组裸鼠肿瘤生存情况和HIF-1α沉默对肿瘤的重量、体积抑制率的影响;采用ELISA方法检测移植瘤组织中HIF-1α干扰后其蛋白表达量的变化;分别利用实时荧光定量PCR、蛋白印迹法检测各组裸鼠移植瘤组织中HIF-1α、CEACAM1及VEGF-C mRNA和蛋白的表达水平.结果:发现HIF-1α干扰组中裸鼠移植瘤的体积及重量均显著小于空白对照组,表明HIF-1α表达沉默显著抑制移植瘤的生长,同时明显下调CEACAM1及VEGF-C的表达.结论:口腔癌中HIF-1α可能通过调节CEACAM1及VEGF-C的表达来影响肿瘤的生长.