乳腺癌组织中ABCG2及CD44+/CD24-细胞表达与预后的关系

摘要

Objective To investigate the expressions of ABCG2 and CD44 +/CD24- cells in breast carcinoma and to discuss their relationship with prognosis. Methods The expressions of CD44+/CD24- cells and ABCG2 were measured by immunohistochemical double-staining and single-staining method respectively in 60 cases of breast carcinoma tissue , 60 cases of para-carcinoma tissue , and 30 cases of breast hyperplasia symptom tissue.Results The positive expression rates of ABCG2 were 50.00％ , 16.67％ , and 60.00％ respectively in breast carcinoma group, para-carcinoma group, and hyperplasia group respectively. And those in hreast carcinoma group and hyperplasia group were significantly higher than that in para-carcinoma group (both P＜0.01). The positive expression rates of CD44+/CD24- cells were 5.00％ , 3.33％ , and 26.67％ respectively in the three groups , and that in hyperplasia group was significantly higher than those in hreast carcinoma group and para-carcmoma group (both P ＜ 0.01). The expressions of ABCG2 and CD44+/CD24- cells were not associated with tumor size , differentiation ,clinical stages , lymphoid metastasis , relapse , and stromal lymph cell reaction ( all P ＞ 0.05 ). There was correlation between the expression of ABCG2 and tumor-free survival period (P ＜ 0.05) , but no correlation between the expression of CD44+/CD24- cells and tumor-free survival period was observed (P＞ 0.05 ). There was no correlation hetween the expressions of CD44+/CD24- cells and ABCG2 (P ＞ 0.05 ). Conclusions ABCG2-negative patients have a longer tumor-free survival period, And the CD44+/CD24- cells were found not only in breast carcinoma tissue but also in para-carcinoma tissue and hyperplasia tissue.%目的:观察ABCG2 以及CD44+/CD24-细胞在乳腺癌中的表达及与预后的关系.方法:应用免疫组化双染技术检测60例乳腺癌、癌旁及30例乳腺增生症组织中CD44+/CD24-细胞的表达并应用单染技术检测ABCG2.结果:ABCG2在乳腺癌、癌旁和增生组织的阳性表达率分别为50.00%、16.67%和60.00%,乳腺癌及增生组织表达均高于癌旁(P ＜ 0.01);CD44+/CD24- 细胞在3组的阳性表达率分别为5.00%、3.33%和26.67%;增生组织表达高于乳腺癌及癌旁(P ＜ 0.01).两者表达与肿瘤大小、病理分级、临床分期、淋巴结有无转移、复发与否、间质有无淋巴反应之间无统计学意义(均P ＞ 0.05);ABCG2表达与无瘤生存期相关(P ＜ 0.05),CD44+/CD24-细胞表达与无瘤生存期不相关(P ＞ 0.05).ABCG2表达与CD44+/CD24-细胞无相关性 (P ＞ 0.05).结论:ABCG2阴性患者无瘤生存期长;CD44+/CD24-细胞并非只存在于乳腺癌组织中.