目的 探讨慢性阻塞性肺疾病(COPD)患者肺泡及外周血单核源性巨噬细胞Cofilin的表达与其吞噬烟曲霉功能的相关性.方法 选取2015年7月至2016年5月南方医科大学珠江医院呼吸内科门诊20名COPD患者,其中观察组1(2011版GOLD中A和B组),观察组2(2011版GOLD中C和D组),健康对照组10人.3组行支气管肺泡灌洗术、采取外周血,分别提取AM和MDM,检测各组的AM和MDM对烟曲霉孢子的吞噬能力.运用实时荧光定量(qRT-PCR)和免疫蛋白印迹实验(Western blot)检测3组Cofilin蛋白的表达,比较其差异.结果 3组人群MDM/AM吞噬烟曲霉孢子的菌落数分别为(17±3)个、(16±2)个、(42±3)个(F=73.446,P<0.001),两观察组与健康组差异明显,而两观察组无明显差异.qRT-PCR和Western blot结果显示,两观察组中Cofilin蛋白表达明显高于健康组.结论 COPD患者肺泡及外周血单核源性巨噬细胞吞噬烟曲霉功能降低可能与Cofilin蛋白升高有关.%Objective To investigate the expression of Cofilin in alveolar and peripheral blood mononuclear cells in COPD patients and the correlation with the function of phagocytic aspergillus. Methods 20 COPD pa-tients were selected from July 2015 to May 2016 in the outpatient department of respiratory medicine of Zhujiang Hospital of Southern Medical University ,then divided into the experimental group 1(group Aand B in 2011 version of GOLD),group 2(test group C and group D in 2011 version of GOLD),and the healthy control group in 10 people. The AM and MDM in the peripheral bloodwere extracted respectively in the 3 groups by bronchoalveolar lavage and,and the ability of AM and MDM in each group were detected. The expression of Cofilin protein was measured by real-time fluorescence quantitative(qRT-PCR) and Western blotting ,and the differenceswere compared among the three groups. Results The colony numbers of MDM/AM in the 3 groups were 17 ± 3,16 ± 2, 42 ± 3(F = 73.446 ,P < 0.001),and the colony numbers of MDM/AM in the two test groupshad significantly different from the healthy group ,while no significant difference was found in the two test groups. The results of fluorescence quantitative reverse transcription polymerase chain reaction and Western blot analysis showed that the expression of Cofilin in the two test groups was significantly higher than that in the healthy group. Conclusion The decreased function of phagocytic aspergillus in the alveolar and peripheral blood mononuclear cells in COPD patients may relates to the increase of cofilin.
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