Using in vitro leaves of Zizyphus jujuba ‘ Kongfusucui’ plantlet as explants, effects of culture method,phytohormone proportion and culture time on induction rate of adventitious shoot from leaves and effects of sucrose concentrations in rooting media on rooting of adventitious shoot were studied. The results show that induction rate of adventitious shoot by two-step culture method ( firstly cultured on medium Ⅰ for four weeks then cultured on medium Ⅱ for three weeks) is significantly higher than that by one-step culture method (cultured continuously on medium Ⅰ for seven weeks). Phytohormone proportion in medium Ⅰ has an obviously influence on induction rate of adventitious shoot, which gradually increases with increasing of TDZ concentration in medium Ⅰ . And adding 1.0 mg · L-1 TDZ in medium Ⅰ leads to induction rate with above 80％. With culture time prolonging ( cultured for 1, 2, 3 or 4 weeks on medium Ⅰ ,respectively), induction rate increases gradually. Sucrose concentration has a significant effect on rooting of adventitious shoot, sucrose with higher concentration (30 g · L-1 ) is beneficial to rooting. According to induction rate and growth status of adventitious shoot, it is determined that optimal culture method of adventitious shoot induction from Z. jujuba ‘ Kongfusucui’ leaves is two-step culture method, that is, leaves firstly cultured on WPM medium containing 1.0 mg · L-1 TDZ and 0.5 mg · L-1 IAA for four weeks, and then cultured on WPM medium containing 0.5 mg · L-1 IAA and 1.0 mg · L-1 GA3 till adventitious shoot produced.%@@ 红枣营养丰富、富含维生素,深受广大消费者喜爱,但由于枣(Zizyphus jujuba Mill.)树在生长过程中经常受到细菌、真菌等病害的危害,制约了红枣的产量.通过品种改良可以获得优良的枣树品种,但由于枣胚败育率高及落花落果严重等因素,导致利用常规杂交育种方法很难达到品种改良的目的.基因工程的外源基因遗传转化技术为植物抗病品种的选育提供了育种新途径,而遗传转化技术的成功应用则主要依赖于植物高效再生体系的建立.因此,建立枣树的高效再生体系是实现其分子育种的首要步骤,对枣树的品种选育至关重要.
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