目的：建立高效液相色谱法测定没食子提取物中三种成分的含量方法。方法色谱柱：Waters Symmetry C18（4.6 mm ×250 mm，5μm）；流动相：甲醇-水（25：75）（冰乙酸调pH至4.5）；流速为1.0 mL·min-1，柱温：30℃，检测波长：273 nm。结果没食子酸、没食子酸甲酯、没食子酸乙酯的回收率分别为99.79%、99.60%、99.45%；线性范围分别为0.2~3.2 μg、0.002~0.16μg、0.0108~0.54μg。结论结果准确，重复性好，可用于该产品的质量控制。%Objective To establish a method for the simultaneous determination of gallic acid,methyl gallate and ethyl gallate from gallnut extracts. MetHods HPLC was used to quantitative analysis. The Waters Symmetry C18(4. 6 mm × 250 mm,5 μm)was used,and mobile phase was composed of methanol-water(25:75)(adjust pH to 4. 5 with acetic acid). The detection wavelength was 273 nm at 30℃. The flow rate was 1. 0 mL·min-1 . Results The gallic acid,methyl gallate and ethyl gallate's spotting recovery were 99. 79%,99. 60%,99. 45%,and the linear ranges were 0. 2~3. 2 μg,0. 002~0. 16 μg,0. 010 8~0. 54 μg,respectively. CoNclusioN The results were accurate and the reproducibility was good. The method can be used for the quality control of the product.
展开▼
