Kinetic performance comparison of fully and superfcially porous particles with sizes ranging between 2.7 lm and 5 lm: Intrinsic evaluation and application to a pharmaceutical test compound

摘要

The reintroduction of superfcially porous particles has resulted in a leap forward for the separation performance in liquid chromatography.The underlying reasons for the higher effciency of columns packed with these particles are discussed.The performance of the newly introduced5 mm superfcially porous particles is evaluated and compared to 2.7 mm superfcially porous and3.5 and 5 mm fully porous columns using typical test compounds(alkylphenones)and a relevant pharmaceutical compound(impurity of amoxicillin).The 5 mm superfcially porous particles provide a superior kinetic performance compared to both the 3.5 and 5 mm fully porous particles over the entire relevant range of separation conditions.The performance of the superfcially porous particles,however,appears to depend strongly on retention and analyte properties,emphasizing the importance of comparing different columns under realistic conditions(high enough k)and using the compound of interest.