目的:新型形状记忆聚合物(SMP)支架用于组织工程修复骨缺损的可行性研究.方法:通过热压成形术合成具有形状记忆功能的支架,应用体视显微镜和扫描电镜观察支架材料形状记忆能力及塑形前后的孔隙变化.建立兔下颌骨骨缺损模型,将塑形后的形状记忆支架材料(SMP支架、BMP-2-SMP支架,并设立空白缺损对照组)植入骨缺损区.通过大体标本观察、Micro-CT检测及HE染色,对比3组骨缺损区域的成骨差异.结果:体视显微镜及扫描电镜结果示,材料塑形后孔径变小,结构更加致密;支架材料在37℃下逐渐恢复到初始形状,具有形状记忆功能.Micro-CT及HE染色显示植入SMP支架后骨缺损区有明显成骨,而支架携带BMP-2后缺损区新生骨量更多,骨质更加成熟.结论:SMP支架可以促进骨缺损的修复,而携载BMP-2可进一步提高该支架对骨缺损区的成骨修复能力,这在组织工程学修复骨缺损中具有巨大的临床应用前景.%Objective:A fesability study of a new smart shape memory polymer (SMP) scaffold in bone tissue engineering. Methods: SMP scaffold synthesized via thermo-compression, its capacity of shape memory and changing of pore observed by stereoscopic microscope and scanning electron microscope (SEM). SMP scaffold was implanted into rabbit mandibular bone defect area. Osteogenic potentials was evaluated by gross observation, Micro-CT examination and HE staining. Results: Stereo microscope and scanning electron microscopy showed pores of SMP scaffolds were smaller, and structure was more compact after scaffolds shaped. SMP scaffolds which performed well capacity of shape memory recovered to the initial shape at 37℃. Micro-CT and histomorphometry examination demonstrated the scaffold promoted osteogenesis of the rabbit mandibular bone defect. BMP-2 improved the osteogenic potential significantly. Conclusion: The SMP scaffold can promote and repair bone defect, and when carrying BMP-2, it can further improve the ability of reconstruction. SMP scaffold demonstrates great potential of application in bone regeneration.
展开▼
