6株旋毛虫49ku ES抗原基因克隆及序列分析

摘要

49 ku ES antigen gene of six different strains of Trichinella spp. Were cloned by RT-PCR, then the cloned sequences and the corresponding sequence of T. Spiralis in GenBank were aligned and compared. The results showed that 49 ku ES gene of Trichinella spp. Were highly conserved, shared high similarities on the levels of nucleotides and amino acids, more than 97.2% and 94.0%, respectively. Therefore, the gene could not well identify different species of Trichinella spp. Encoded protein analysis showed the identity in antigenicity of the six strains, suggesting just one strain the recombinant antigen could be used to detect antiserum against P49 protein of different Trichinella spp.%应用RT-PCR方法从不同来源的6株旋毛虫肌幼虫总RNA中克隆49 ku ES抗原基因序列,与GenBank中T.spiralis相应序列进行比对分析.结果表明,旋毛虫49 ku ES基因具有相当强的保守性,不同虫株核苷酸和氨基酸序列的同源性分别达97.2％和94.0％以上,序列间差异很小,不能较好地区分各旋毛虫种；该基因编码蛋白的抗原性很稳定,不同旋毛虫49 ku ES抗原性几乎没有差别,只要获得一个虫株的重组49 ku ES蛋白,即可用于其他不同种株旋毛虫病的免疫诊断及预防.