MAGE－4抗原 MHC－I／MHC－II 限制性表位肽的预测

摘要

Objective To predict the MHC-I/MHC-II restricted epitope peptide of MAGE-4. Methods The amino acid of MAGE-4 was retrieved by NCBI protein datebase.Based on this,HLA-A*0201 restricted epitope peptide was predicted by BIMAS、SYFPEITH1 and IEDB on line service. Then we used SYFPEITHI and NetMHCIIpan 3.1 to predicted MHC-II restricted peptide.Results Three peptides were obtained,which contain MHC-I restricted epitope as well as MHC-II restricted epitope.Conclusion This result provide a basis for choosing peptides in order to immunotherapy of colorectal cancer.%①目的预测黑色素瘤相关抗原－4（MAGE－4）抗原的 MHC－I／MHC－II 限制性表位肽。②方法利用 NCBI 蛋白数据库检索到 MAGE－4抗原的氨基酸序列，BIMAS、SYFPEITH1、IEDB 等3种方案分别对 MAGE－4抗原的 HLA－A*0201限制性 MHC－I 类抗原表位进行预测，选出候选肽；然后利用 SYFPEITHI 服务器和 NetMHCIIpan 3．1服务器对候选肽周围的 MHC－II 限制性表位进行预测。综合分析选出 MHC－I／MHC－II 限制性表位肽。③结果筛选得到3条肽段，既存在 MHC－I 限制性表位又存在 MHC－II 限制性表位肽。④结论为 MAGE－4抗原用于结肠癌免疫治疗肽段的选择提供了依据。