△Np63和TAp63在银屑病不同发展时期的表达

摘要

Objective To investigate the expression of△Np63 and TAp63 mRNA and proteins in different stages of epidermal keratinocytes of psoriasis.Methods Clinical psoriasis lesions specimens in different stages were collected,the expression of △Np63 and TAp63 mRNA was detected by Real-time PCR of absolute quantification.△ Np63 protein expression was detected by Immunohistochemical method.Results Compared with normal skin tissues andskin lesions tissues,TAp63 mRNA was significantly down-regulatedat active stage,resting stage and plateau stage(P＜0.05),while expression rate showed no difference between the three groups(P＞0.05).△ Np63 mRNAwas significantly up-regulated at active stage,resting stage and plateau stage (P＜0.05).The expression of△Np63 mRNA was significantly increased in the progression period than that in the stationary phase and the stable stage (P＜0.05).Immunohistochemical results showed that △ Np63 protein was no significant difference in puberty quiescence (P＞0.05),and significantly up-regulatedat active stageand plateau stage (P＜0.05),compared with normal tissue and lesion tissue.Conclusion The effect of p63 gene on psoriasis was closely related to the expression of different subtypes.%目的 探讨△Np63和TAp63 mRNA及其蛋白在寻常银屑病不同发展时期的表达.方法 收集临床银屑病进展期、静止期、稳定期皮损组、皮损旁组、以及正常组,用Real time PCR检测△Np63和TAp63 mRNA的表达;免疫组化法检测△Np63蛋白在正常组和银屑病进展期、静止期、稳定期皮损组的表达情况.结果 荧光定量PCR结果显示,相比于正常组和皮损旁组,TAp63 mRNA在银屑病进展期、静止期、稳定期皮损组的表达降低(P＜0.05),进展期、静止期、稳定期组间表达率差异无统计学意义(P＞0.05).而△4p63 mRNA在银屑病进展期、静止期和稳定期表达升高(P＜0.05),进展期、静止期、稳定期组间显示进展期升高(P＜0.05).免疫组化结果显示,相比于正常组和皮损旁组,△Np63蛋白在银屑病静止期表达差异无统计学意义(P＞0.05),而在进展期和稳定期中高表达(P＜0.05).结论 p63基因对银屑病的作用与其不同亚型△Np63与TAp63的表达密切相关.