目的:了解江苏省HIV-1流行株env和pol基因亚型及其系统.方法:收集42份血清抗-HIV阳性者的血浆标本并提取RNA,逆转录为模版DNA后采用巢式 PCR法扩增env及 pol基因,对扩增产物进行核苷酸序列测定,比对确定基因亚型,分析不同途径感染者的基因亚型,构建系统进化树.结果:42份标本中,扩增出env及pol基因片段27份(64%);共发现5种 HIV-1亚型,其中 CRF01-AE亚型 19份、C亚型 2份、B亚型3份、CRF08-BC 2份、G亚型1份.经静脉药瘾感染者基因型多为CRF01-AE亚型;经性接触感染者HIV-1基因亚型包括检出的全部亚型,经性接触及吸毒感染者基因亚型均为CRF01-AE亚型.两基因构建系统进化树高度一致,主要有CRF-01AE、B-07、G、C和CRF-08BC 5种HIV亚型在流行,而以CRF-01AE为主要流行株.结论:江苏省 HIV-1基因亚型至少有5种,其中 CRF01-AE为主要亚型.%Objective; To monitor the epidemic subtypes and evolution of HIV -4 in Jiangsu province by analyzing pol and env gene. Methods; The env and pol genes were amplified by nested PCR following RNA reverse transcription extracted from 42 HIV-1 positive plasma samples. The amplicons were sequenced , and the genetic subtype was identified by comparative analysis. The phylogenetic tree was constructed by ME methods. Results: Env and pol gene were successfully amplified from 27 samples, including 19 CRF01_A E subtype , 3 B subtype, 2C subtype, 2 CRF08_BC and 1 G subtype. CRF01-AE subtype was found in drug users by intravenous injection ;all the subtypes were detected in the sexual contact ; the CRF01-AE subtype was detected between the sexual contact and drug users at the same time. The phylogenetic tree constructed by env and pol were highly consistent , mainly CRF_01AE, B_07 , G, C and CRF-08BC. And 5 HIV-1 subtypes were determined, Conclusion; Therewere at least 5 HIV-1 gene subtypes in AIDS patients in Jiangsu province , and CRF01_AE subtype prevailed.
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