目的 研究持续光照对大鼠生殖内分泌及行为学的影响,探讨滋阴补阳方序贯疗法对持续光照大鼠的干预作用.方法 采用持续光照的方法,建立大鼠光照节律紊乱模型.正常对照组大鼠给予12/12h光/暗循环的光照条件,将造模大鼠随机分为模型组和中药组.对照组、模型组予以生理盐水灌胃,中药组予以滋阴补阳方序贯治疗.各组大鼠治疗后进行强迫游泳实验及糖水偏好实验,并进行比较不动时间和糖水偏好值.治疗后对血清雌二醇(E2)、卵泡刺激素(FSH)、黄体生成素(LH)、促性腺激素释放激素(GnRH)、褪黑素(MT)、5-羟色胺(5-HT)进行测定,并进行组间比较.治疗后对卵巢组织进行电镜下观察,比较各组大鼠卵巢超微结构的差异.结果 在糖水偏好实验中,模型组和中药组大鼠的糖水偏好值明显低于对照组,中药组大鼠糖水偏好值较模型组高.在强迫游泳实验中,模型组和中药组大鼠的不动时间高于对照组,中药组大鼠不动时间较模型组低.模型组和中药组较对照组E2、FSH、LH、MT水平低,而且中药组在FSH、MT水平上明显高于模型组,但E2、LH水平与模型组未见明显差异.与此同时,模型组较对照组5-HT水平高,中药组5-HT水平明显低于模型组,而对照组和中药组间未见显著差异.模型组和中药组的卵巢较对照组从超微结构看可见有细胞损伤,模型组较中药组损伤更加严重.结论 持续光照会引起大鼠情绪障碍,生殖功能受到抑制,性激素水平失调,滋阴补阳方序贯疗法可在一定程度改善大鼠情绪以及生殖功能.%OBJECTIVE To discuss the effect of continuous illumination on reproductive endocrine and behavior of rats and intervention effect of Nourishing Yin and Tonifying Yang Fang sequential traditional Chinese medicine.METHODS Normal control group rats were under the light condition of 12/12 h light/dark cycle.Model group rats were under the light condition of 24h continuous illumination.Light intensity were chosen (300±20)lux.The time of building model was 50 days.The model assessment was according to the situation of estrus cycles.After eliminating unsuccessful models,residual model rats were randomly divided into model group and traditional Chinese medicine group.Control group and model group were given saline lavage.Traditional Chinese medicine group was given Nourishing Yin and Tonifying Yang Fang sequential traditional Chinese medicine treatment.Three groups of rats had forced swimming test and the sugar water preference experiments after the treatment.They were compared the fixed time and sugar water preference values.Three groups of rats were measured serum E2(Estradiol),FSH(Follicle Stimulating Hormone),LH(Luteinizing Hormone),GnRH(Gonadotropin-Releasing Hormone),MT(Melatonin) and 5-HT(5-Hydroxy Tryptamine) after the treatment and compared between the three groups.The ovarian tissues of three groups,rats were observed under the electron microscope after the treatment.The difference of ovarian ultrastructure was compared among the three groups.RESULTS In the sugar water preference experiments,the sugar water preference values of the model group and the traditional Chinese medicine group were obviously lower than the control group.The sugar water preference values of the traditional Chinese medicine group rats were higher than the model group rats.In the forced swimming test,the fixed time of the model group and the traditional Chinese medicine group rats was higher than the control group rats.The fixed time of the traditional Chinese medicine group rats was lower than the model group rats.The level of E2(Estradiol),FSH(Follicle Stimulating Hormone),LH(Luteinizing Hormone) and MT(Melatonin) of the model group and the traditional Chinese medicine group were lower than the control group.The level of FSH(Follicle Stimulating Hormone) and MT(Melatonin) of the traditional Chinese medicine group were obviously higher than the model group.However,there was no difference between the traditional Chinese medicine group and the model group on the level of E2(Estradiol) and LH(Luteinizing Hormone).At the same time,the level of 5-HT(5-Hydroxy Tryptamine) of the model group was higher than the control group.The level of 5-HT(5-Hydroxy Tryptamine) of the traditional Chinese medicine group was significantly lower than the model group.However,there was no significant difference between the control group and the traditional Chinese medicine group.From the ultrastructure angle,there was visible damage in the model group and the traditional Chinese medicine group of ovarian cells compared with the control group.Furthermore,the injury of the model group was more serious than the traditional Chinese medicine group.CONCLUSION Continuous illumination may lead to mood disorders of rats,restraining the reproductive function and sex hormone level disorders.Nourishing Yin and Tonifying Yang Fang sequential traditional Chinese medicine can improve the mood and reproductive function of rats in a certain degree.
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