MAPK蛋白磷酸化与结直肠癌患者临床病理和生存率的相关性研究

摘要

目的：研究结直肠腺癌患者MAPK蛋白磷酸化表达与临床病理及生存率的相关性。方法收集经病理科诊断、手术切除的原发结直肠腺癌患者的癌组织标本166例及其癌旁正常组织标本61例。采用免疫组织化学SABC染色方法检测P－ERK1／2蛋白磷酸化表达情况，并对病例进行临床病理资料数据统计和随访患者6～60月生存情况。结果（1）结直肠癌患者中61.45％癌组织P－ERK1／2蛋白表达高磷酸化；98.36％癌旁正常肠组织无磷酸化。（2）癌组织P－ERK1／2蛋白高磷酸化女性较男性患者表达率高，癌组织原发部位于结肠的P－ERK1／2蛋白高磷酸化较直肠癌的患者表达率高，两者差异有统计学意义（ P ＜0.05）；癌组织P－ERK1／2蛋白磷酸化高表达与患者年龄、临床分期、浸润深度、淋巴转移、组织分化及远处转移无相关性（ P ＞0.05）。（3）癌组织高磷酸化较无磷酸化患者的生存率低，与患者的生存率成负相关（ P ＜0.05）。（4）癌组织标本P－ERK1／2蛋白高磷酸化与无磷酸化患者1a、3a、5a死亡率比较，5a的患者死亡率两者差异有统计学意义（ P ＜0.05）。结论癌组织P－ERK1／2蛋白表达高磷酸化，癌旁正常肠组织几乎无磷酸化；癌组织中P－ERK1／2蛋白表达高磷酸化与患者性别、肿瘤原始发病部位有显著性差异；癌组织P－ERK1／2蛋白高磷酸化与患者生存率呈负相关，P－ERK高磷酸化患者生存时间短、预后差。%Objective To study the Correlation between the expression of MAPK protein phosphorylation and clinical pathologi -cal and survival rates in colorectal cancer patients.Methods Collected 166 samples of rectal cacer tissues and pararectal cancer tis-sues from affiliated hospitals of Jiamusi University during 2007-2009.The expression of phosphorylated P -ERK1/2 was detected by SABC method.Analysis of clinical pathological data of these patients and follow -up patient ’ s survival status from 6 to 60 months were conducted.The relationship between phosphorylated P -ERK1/2 and clinical pathological characteristics , survival status.Results A-mong colorectal cancer patients , there are 61.4%(102)expressed higher phosphorylated P -ERK1/2, and very few phosphorylated P-ERK1/2 expressed in the para -cancer tissues.The phosphorylated P -ERK1/2 protein in cancer tissues of females is higher than that of males ( P <0.05).The primary cancer which located at colon expressed higher phosphorylated P -ERK1/2 than that located at rectum.The expression of phosphorylated P -ERK1/2has no relationship with age, position, size, clinical stage, lymphatic metas-tasis, differentiation, pathological grade and survival rate ( P >0.05).There are negative relationship between high phosphorylated P-ERK1/2 and survival rate in first year , third year and fifth year ( P <0.05).Conclusion The expression of high phosphorylated P-ERK1/2in rectal cancer has relationship with gender and primary cancer location.The expression of high phosphorylated P -ERK1/2 has negative relationship with survival rate.