Live-cell single-molecule imaging of the cytokine receptor MPL for analysis of dynamic dimerization

摘要

Dear Editor,MPL is a cytokine receptor that regulates the production of myeloid cells,in particular megakaryocytes and platelets(Kaushansky,2005).Dimerization of MPL is important not only for the initiation of signalling(Kaushansky,2005)but also for the resistance to a recent therapy for myeloproliferative neoplasms(Koppikar et al.,2012).In the current model,cytokine receptors exist as a mixture of monomers and dimers without ligand stimulation,while ligand binding induces additional receptor dimerization(Kaushansky,2005).