启东肝癌高发区肝癌患者血清中p53突变研究

摘要

Objective To investigate plasma p53 mutation in hepatocellular carcinoma(HCC)patients from Qidong,and to define its significance. Methods We collected blood samples from 25 hepatocellular carcinoma patients,20 cirrhotic patients and 30 healthy controls in Qidong area. DNA was extracted and purified from 200μ1 of plasma from each sample. The 249[Ser]p53 mutation was detected by restriction digestion analysis and direct sequencing of exon-7 PCR products. Results We found in exon 7 of p53 gene G→T transversion at the third base of 249 codon resulting 249[Arg]→249[Ser]mutation in 11/25(44%)hepatocellular carcinoma cases, 4/20 (20%) cirrhotics, and 2/30(7%) healthy controls.(p<0.01). Conclusion The 249[Ser]P53 mutation in plasma is strongly associated with hepatocellular carcinoma in Qidong patients and the mutation should be developed into a new early diagnostic marker for HCC.%目的研究启东地区肝癌患者血清中p53基因突变,并确定其对肝癌诊断的意义.方法收集25例肝癌,20例肝硬化,30例健康对照者的血标本,提取DNA,应用限制性酶切及直接序列分析方法测定p53基因第七外显子突变.结果显示p53基因第七外显子的249密码子产生突变,ARG变为SER,肝癌,肝硬化,健康者中发生率分别为44%(11/25),20%(4/20),7%(2/30) (P＜0.01).结论启东地区肝癌患者血清中p53突变与肝癌发生密切相关,其可作为新的肝癌早期诊断标志.