奥曲肽对伊立替康化疗后迟发性腹泻小鼠肠黏膜损伤的治疗作用

摘要

Objective:To observe the treatment effectiveness of mucositis of Irinotecan induced delayed diarrhea in mice.Methods:Thirty healthy Kunming mice were randomized into 3 groups:Group A:experimental group of octreotide therapy;Group B:control group of CPT-11 induced diarrhea;Group C:injection of physiological saline as control group.To observe the condition of delayed-onset diarrhea,sacrifice the mice on the 9th day,collect the small intestines and colons and evaluate the mucositis according to Chiu criteria under light microscope.Results:Octreotide could release the symptom of CPT-11 induced delayed-onset diarrhea compared with the control group,mice of CPT-11 induced diarrhea in octreotid treatment group release diarrhea symptom,the score decreased obviously and the condition of weight loss comes to alleviation.(P ＜ 0.05).Octreotide could improve the injuries of intestinal mucosa caused by CPT-11 induced delayed-onset diarrhea.Compared with the control group,mice of CPT-11 induced diarrhea in octreotide treatment group alleviated intestinal mucositis (P ＜ 0.05).Conclusion:To some extent,octreotide could treat irinotecan induced delayed-onset diarrhea and alleviate intestine and colon mucositis.%目的:观察和探索奥曲肽对伊立替康化疗后迟发性腹泻小鼠肠黏膜损伤的治疗作用.方法:健康昆明小鼠30只,随机分成3组:A组:CPT-11致小鼠腹泻奥曲肽治疗组；B组:CPT-11致小鼠腹泻对照组；C组:腹腔注射等量生理盐水作为空白对照.观察迟发性腹泻发生情况,实验开始后第9天将小鼠处死,取小肠及结肠组织.光镜下根据Chiu标准对肠黏膜损伤程度进行分级.结果:与对照组相比,治疗组小鼠伊立替康引起的迟发性腹泻症状明显减轻,肠黏膜损伤程度亦减轻(P＜0.05).结论:奥曲肽可以对伊立替康引起的迟发性腹泻起到一定程度的治疗作用.