去氢骆驼蓬碱衍生物DH-332对人结肠癌细胞株HCT116体外侵袭能力的影响

摘要

Objective:To investigate the effect on the invasive of human colon cancer cell line HCT116 induced by harmine derivative DH-332 in vitro.Methods:Human colon cancer cells HCT116 were treated with different concentrations of DH-332,and the migration and invasion ability of HCT116 in vitro were determined by scratchwound assay,cell adhesion assay,and Transwell invasion assay.Results:After treatment with different doses of DH-332 in HCT116 cells,scratch-wound assay shows cell migration ability was lower than the control group,cell adhesion assay and Transwell invasion assay showed that the ability of adhesion and invasion descended with dose and time dependent.(P ＜ 0.05).Conclusion:Harmine derivative DH-332 can inhibit the invasion ability of human colon cancer cells HCT116 in vitro.%目的:探讨去氢骆驼蓬碱衍生物DH-332对人结肠癌细胞株HCT116体外侵袭能力的影响.方法:不同浓度去氢骆驼蓬碱衍生物DH-332作用于人结肠癌细胞株HCT116后,采用细胞划痕,细胞黏附,Transwell侵袭实验检测去氢骆驼蓬碱衍生物DH-332对HCT116细胞体外迁移和侵袭力的影响.结果:不同浓度去氢骆驼蓬碱衍生物DH-332作用于HCT116细胞后,细胞划痕实验显示细胞迁移能力较对照组低；细胞黏附实验和Transwell侵袭实验显示DH-332作用后,细胞的黏附,侵袭能力降低,并呈时间和剂量依赖性(P＜0.05).结论:去氢骆驼蓬碱的衍生物DH-332可以抑制人结肠癌细胞HCT116体外侵袭能力.