白花蛇舌草总黄酮对肝癌MHCC97-H细胞增殖的抑制作用

摘要

目的：观察白花蛇舌草总黄酮（FOD）对肝癌 MHCC97- H 细胞增殖和凋亡的影响。方法：将生长良好的细胞分为空白组、FOD 组、5- FU 组及联合组四组，分别干预48小时，观察细胞的形态，MTT 法检测其对肿瘤细胞增殖的抑制作用；流式细胞仪检测 FOD 对 MHCC97- H 肝癌细胞凋亡的影响。结果：FOD 对肝癌MHCC97- H 细胞有显著的抑制作用，并且具有剂量和时间依赖性，不但能使细胞密度减少，细胞变小，核固缩，而且能显著抑制人肝癌 MHCC97- H 细胞的增殖并诱导该细胞的凋亡（P ＜0.05）。结论：白花蛇舌草总黄酮能够通过抑制肿瘤细胞生长、促进肿瘤细胞凋亡，明显抑制肝癌 MHCC97- H 细胞的增殖。%Objective:To observe the effect of total flavonoids of Oldenlandia diffusa(FOD)on the proliferation and apoptosis of hepatocellular carcinoma MHCC97 - H cells. Methods:MHCC97 - H cells were divided into four groups:control group,FOD group,5 - FU group and combined group,intervented for 48 hours,observe the cells form, detect the inhibitory effect on tumor cell proliferation by MTT method and the influence of FOD on apoptosis of hepa-tocellular carcinoma cell line MHCC97 - H by flow cytometry. Results:FOD had significant inhibitory effect on liver cancer MHCC97 - H cells with dose and time dependence,not only can make the cells density decreased,cells be-come small,nuclear pyknosis,and inhibit the apoptosis of hepatocellular carcinoma MHCC97 - H cells proliferation and induce cell apoptosis(P < 0. 05). Conclusion:Total flavones of Oldenlandia diffusa willa can inhibit the prolifer-ation of hepatocellular carcinoma MHCC97 - H cells by inhibiting the growth of tumor cells and promoting the apopto-sis of tumor cells.