外周T细胞淋巴瘤患者中PD-1的表达对CD45RA+、CD45RO+T细胞的影响

摘要

Objective:To investigate the relationship between the expression of PD-1 in lymphoid tissue and the level of initial and memory T cells in peripheral blood in peripheral T cell lymphoma (PTCL) patients.Methods:The expression of PD-1 was detected by immunohistochemistry in lymphoma tissue in 20 PTCL patients.The proportion of CD4+CD45RA+,CD4+CD45RO+,CD8+CD45RA+ and CD8+CD45RO+T cells before and after CHOP chemotherapy in peripheral blood in 20 PTCL patients were detected by flow cytometry.The relationship between expression of PD-1 and T cell subsets was analysed.Results:The expression of PD-1 and PD-L1 protein were increased in PTCL patients,and PD-1 positive patients had poor curative effect.The CD4+T cells and CD8+T cells in PD-1 positive patients were significantly lower than that of PD-1 negative group.The CD4+CD45RO+,CD8+CD45RA+ and CD8+CD45RO+T cells in the PD-1 negative group were significantly higher than those in the PD-1 positive group,the difference was statistically significant.Conclusion:The expression of PD-1/PD-L1 protein in patients with PTCL is abnormal,and the PD-1 positive patients have more obvious defects in T cell immune function.%目的:探讨外周T细胞淋巴瘤(PTCL)患者淋巴瘤组织中PD-1的表达与外周血中初始和记忆T细胞水平的关系.方法:20例PTCL患者采用免疫组化法检测淋巴瘤组织中PD-1的表达,采用流式细胞术检测CHOP方案化疗前后外周血中CD4+CD45RA+、CD4+CD45RO+、CD8+CD45RA+和CD8+CD45RO+T细胞的比例,分析PD-1的表达与T细胞亚群的关系.结果:PD-1、PD-L1蛋白在PTCL患者中表达升高,PD-1阳性患者疗效较差.PD-1阳性患者中CD4+T细胞、CD8+T细胞明显低于PD-1阴性患者;PD-1阴性组患者CD4+CD45RO+、CD8+CD45RA+、 CD8+CD45RO+T细胞明显高于PD-1阳性患者,差异有统计学意义.结论:PTCL患者中存在PD-1/PD-L1蛋白的表达异常,PD-1阳性患者存在更明显的T细胞免疫功能缺陷.