美洲大蠊提取液对溃疡性结肠炎大鼠结肠黏膜 IGF-1、TGF-β表达的影响

摘要

Objective To investigate the effects of American cockroach extract (ACE) enema on the expression of in-sulin-like growth factor-1 (IGF-1) and transforming growth factor-β (TGF-β) in the colonic mucosa of rats with ulcerative colitis (UC) induced by 2,4,6-trinitrobenzene sulphonic acid(TNBS). Methods Thirty healthy male Wistar rats were randomly divid-ed into the control group(8 cases) and model group(22 cases) according to the random number table method in September 2015. Six cases in the model group were randomly killed by the random number table method after constructing the UC model by TNBS. The colonic changes were observed under the microscope for confirming the model construction success ,other 16 cases were di-vided in to the research group and model group according to the random number table method ,8 cases in each group. The re-search group was given ACE 5 mL/(kg·d)by retention enema ,while the model group and control group were given the same vol-ume of normal saline by the retention enema,once daily for continuous 10 d. The disease activity index (DAI) was calculated after 10 d enema,colonic gross morphology and histological damage scores were performed. The expressions of IGF-1 and TGF-βwere detected by real time reverse transcription-polymerase chain reaction(RT-PCR) and immunohistochemistry. Results Compared with the model group,DAI,colonic gross morphology and histological damage scores in the research group were improved signifi-cantly(P<0.05),the expressions of IGF-1and TGF-βin the research group were significantly lower than those in the model group, the difference was statistically significant ( P<0 . 05 ) . Conclusion ACE has better curative effect in the TNBS induced UC rats,one of possible mechanisms is down-regulating the expression of IGF-1 and TGF-βin colonic mucosa.%目的观察美洲大蠊提取液（ACE）灌肠对2，4，6-三硝基苯磺酸（TNBS）诱导的大鼠溃疡性结肠炎（UC）的疗效和对肠黏膜胰岛素样生长因子-1（IGF-1）、转化生长因子-β（TGF-β）表达的影响。方法2015年9月将30只健康雄性Wistar大鼠采用随机数字表法分为对照组（8只）和模型组（22只），模型组用TNBS建立UC模型后采用随机数字表法随机处死6只，镜下观察结肠变化，确定造模成功，其余16只采用随机数字表法分为研究组和模型组，每组8只。研究组给予ACE 5 mL/（kg·d）保留灌肠，模型组、对照组给予等容积生理盐水保留灌肠，每天1次，连续给药10 d。灌肠10 d后计算疾病活动指数（DAI）、结肠大体形态及组织学损伤评分，采用实时荧光聚合酶链反应、免疫组织化学SP法检测结肠IGF-1、TGF-β的表达。结果研究组大鼠DAI、结肠大体形态及组织学损伤评分均较模型组显著改善，IGF-1、TGF-β表达均明显低于模型组，差异均有统计学意义（P<0.05）。结论 ACE对TNBS诱导的UC大鼠具有较好的治疗作用，可能机制之一是下调结肠黏膜IGF-1、TGF-β的表达。