药用真菌桑黄液体培养基配方的优化

摘要

研究液体培养基配方对桑黄菌丝体生物量和胞外多糖产量的影响。采用四因素三水平的正交试验方法。适宜于菌丝体生物量和胞外多糖产量的配方为A3 B1 C3 D2（麦麸10％，蛋白胨0．5％， KH2 PO40．35％， MgSO40．15％）， pH 6，菌丝体生物量和胞外多糖总产达到1．0077g／100 mL。优化的配方可以用于工业生产获得更多的菌丝体生物量和胞外多糖。%The influence of liquid medium formula on biomass of Phellinus igniarius mycelium and the yield of exocel-lular polysaccharide were studied adopting four factors and three levels orthogonal experiment.The suitable formula for mycelium biomass and exocellular polysaccharide yield of P.igniarius was A3 B1 C3 D2 ( wheat bran 10%, peptone 0.5%, KH2PO4 0.35%, MgSO4 0.15%), pH 6.The total yield of mycelium biomass and exocellular polysaccharide yield was 1.007 7 g/100 mL.The optimized formula can be used in industrial setting in order to get more yield of P. igniarius mycelium biomass and exocellular polysaccharide.