门多萨假单胞菌 NK－01合成中长链聚羟基脂肪酸酯的发酵条件优化

摘要

In order to improve the accumulation of PHAMCL in vivo, single factor and orthogonal experiments were a-dopted to study the optimization fermentation conditions for Pseudomonas mendocina NK-01.When PHAMCL output was taken as an index, under optimized fermentation conditions at 15 g/L glucose, with C/N ratio at 50 and fermented for 48 h, produced over 0.8/L of PHAMCL .When the index was turned to PHAMCL percentage content of dried cell weight ( DCW) , the best fermentation conditions were 10 g/L glucose, with C/N ratio at 60 and fermented for 48 h, the PHAMCL output was over 50% of DCW.This study will provide theoretic foundations for large scale production of PHAMCL by P.mendocina NK-01.%为了提高PHAMCL在门多萨假单胞菌NK－01中的积累，采用单因素实验和正交实验确立了发酵生产PHAMCL的最佳条件，即以PHA产量为指标的最佳发酵条件为15 g／L葡萄糖浓度、C／N＝50、发酵时间48 h，该条件下获得产量0．8 g／L以上的PHA；以PHA占菌体干重百分含量为指标的最佳发酵条件为10 g／L葡萄糖浓度、C／N＝60、发酵时间48 h，该条件下获得占菌体干重50％以上的PHA。该研究将为门多萨假单胞菌NK－01用于PHAMCL的规模化生产提供理论依据。