迷迭香不同生长周期中迷迭香酸的积累动态研究

摘要

目的：研究不同采收期迷迭香中迷迭香酸的积累动态规律.方法：样品采用超声提取，高效液相色谱法测定迷迭香不同采收季节中迷迭香酸的含量.色谱柱：PLATISILTM ODS（250 mm ×4.6 mm，5μm），流动相为V （0.1％磷酸溶液）：V（甲醇）＝（46：54），流速：0.8（ ml/min），检测波长为330 nm，柱温为35℃.结果：待测组分与其他组分分离度良好（R﹥1.5），迷迭香酸在0.348μg~6.96μg范围内呈良好的线性关系（r＝0.9999）；平均回收率为99.96％，RSD 为0.09％.迷迭香酸在一年生长期中的含量随着季节变化存在较为规律的梯度变化趋势，从1月开始，呈现出先降低-升高-降低-升高-降低-升高的趋势，在新芽生长期含量较低，秋冬季平均含量高于春夏季.结论：迷迭香酸的含量随植物生长期不同而变化，实验结果为迷迭香中迷迭香酸的含量测定及药材质量控制提供实验依据.%Objective:To study the accumulation dynamics of rosmarinic acid during a whole yearly period of Rosmarinus officinalis L. . Methods:Rrosmarinic acid was obtained by ultrasonic extraction,and its content was determined by HPLC. The column was PLATISILTMODS(250 mm × 4. 6 mm,5μm). 0. 1% phosphoric -metha-nol(volume ratio of 46:546)was used as the mobile phase at a flow rate of 0. 8 ml/min. The UV detective wavelength was set at 330 nm,and the column temperature was set at 35℃. Results:Rosmarinic acid was well i-solated from other components,and the calibration curve showed a good linear correlation at 0. 348μg~6. 96μg. The average recovery was 99. 96%(RSD = 0. 09%). With the seasons change,the content of rosmarinic acid presents a regular gradient change in a yearly growing period. Since January,the content of rosmarinic acid pres-ents a trend of reducing - increasing - reducing- increasing-reducing- increasing. The content of rosmarin-ic acid is low in new buds growing periods,and the average content in autumn and winter was higher than that in spring and summer. Conclusion:The content of rosmarinic acid has a regular change with the change of growing periods. The experimental results provide experimental basis for the content determination of rosmarinic acid in Rosmarinus officinalis L. and the quality control of herbs.