EML4-ALK融合基因与EGFR基因突变在肺腺癌中的表达

摘要

Objective To investigate the relation between EML4-ALK fusion gene and EGFR gene positive expression in clinical and pathological characteristics.Methods The study applied FISH and the ARMS technology,with detection of the EML4-ALK and EGFR gene in 60 patients from 2008 to 2011 in Tongling People's Hospital with pathologically confirmed lung adenocarcinoma cancer tissues.Results In histopathology of lung adenocarcinoma,EML4-ALK fusion gene positive expression rate were 8.33％.The EGFR gene positive expression rate were 61.67％.The mutation rate of 76.92％ (20/26)in the female patients was higher compared with male mutation rate of 50.0％ (17/34),and the P values was 0.034.the mutation rate of patients with smoking ＜ 400 years package of[74.29％(26/35)] was higher compared with smoking ≥ 400 mutation rate [44.0 ％ (11/25)],and the P values was 0.017.the mutation rate in the ≥60-year-old patients[42.86％ (12/28)]was lower compared with ＜60-year-old mutation rate[78.13％ (25/32)],and the P values was 0.005.All the difference havd statistically significace.The EML4-ALK and EGFR of correlation analysis showed correlation(R =-0.258,P =0.046 ＜0.05),and the difference was statistical.Conclusion EML4-ALK positive expression was lower compared with EG-FR.The expression of EGFR and EML4-ALK showed correlation and the difference had statistical significant.%目的 探讨肺腺癌EML4-ALK融合基因与EGFR基因突变的发生率及临床病理特征.方法 采用FISH和ARMS方法对安徽省铜陵市人民医院2008～2011年手术切除和经皮肺穿刺活检病理确诊的肺腺癌60例石蜡包埋组织分别进行EML4-ALK融合基因和EGFR基因突变检测,并分析与临床病理特征的相关性.结果 肺腺癌病理组织中EML4-ALK融合基因阳性表达率为8.33％,EGFR基因突变率61.67％,女性患者突变率76.92％(20/26)较男性患者突变率50.0％(17/34)高,P=0.034;吸烟＜400年支突变率74.29％(26/35)较吸烟≥400支突变率44.0％(11/25)高,P=0.017;≥60岁患者突变率42.86％(12/28)较＜60岁患者突变率78.13％(25/32)低,P=0.005,差异均有统计学意义.EML4-ALK和EGFR在肺腺癌患者中表达的相关性分析,显示二者之间呈负相关(R=-0.134,p=0.308),差异无统计学意义.结论 EML4-ALK在肺腺癌中低表达,EGFR基因突变高表达,两者呈负相关.