应用正交试验设计研究烟草中总氮测定的消化条件

摘要

To determine the total amount of Nitrogen in tobacco by orthogonal table L9 （34） , and the results showed that the range of digesting condition to the digesting effect of the tobacco samples was： sample weight 〉 the amount of HESO4 〉 the amount of K2SO4 〉 the amount of CuSO4. The sample weight, the amount of K2SO4 , and the amount of H2SO4 have obvious effects except for the amount of CuSO4. The best digesting condition was that temperature was 400 ℃ , time was 180 minutes, the amount was respectively ： the sampie weight 0. 3 g, K2SO4 3.0 g, H2SO4 5mL, and Cu SO4 0. 1 g.%采用L9（3^4）正交设计研究烟草中总氮测定的消化条件．结果表明：消化条件对烟样消化效应的极差是，称样量〉硫酸〉硫酸钾〉硫酸铜；称样量、硫酸和硫酸钾用量对烟样消化效果表现显著，硫酸铜用量不显著；选用400℃、180in、称样量0．3g、硫酸钾3．0g、硫酸5mL、硫酸铜0．1g为最佳消化条件．