芡实组培快繁无菌体系的建立和优化

摘要

以芡实的单芽茎段为外植体进行离体培养和植株再生的优化研究.结果表明，以MS+6-BA1.5mg／L+NAA0.2mg／L作增殖继代培养基为宜，以在MS培养基+蔗糖20g/L+琼脂5.0g/L中添加6-BA （0.1mg/L）+NAA（0.1mg/L）作生根培养基为宜，生根率最高.适当浓度的6-BA对芡实的不定芽增殖有明显的促进作用，6-BA和NAA配合使用可促进芽苗生长，对改善芡实植株组培苗质量有较好的作用.%Stern segments with a single bud of Euryale ferox were used as explants in culture in vitro and for rapid propagation.The results indicated that MS+ 6-BA 1.5 mg/L+NAA 0.2 mg/ L was a suitable medium for shoot multiplication,and the best medium for rooting was MS+ 6-BA(0.1mg/L)+NAA(0.1mg/L) used in proper concentrations promoted shoot multiplication but inhibited stem elongation.Combined application of 6-BA and NAA promoted the elongation of the stem segments.