不同剂量钴对大鼠心肌细胞培养液中心肌酶活性的影响

摘要

Objective:To find the threshold value concentration of cobalt causing myocardium cell damage.Methods:Cobalt in different concentrations (10 μg/ml,20 μg/ml,40 μg/ml,80 μg/ml) were added into the medium of cultured myocardium cell of rat (1～3 days old) in vitro.Then the activities of GOT,CK,LDH,HBDH in the medium were measured.Results:The results showed that when cobalt concentration was 10～20 μg/ml,the activity of GOT,CK,LDH,HBDH did not change markedly.But when cobalt concentration was more than 40 μg/ml,the activities of those enzymes increased significantly.Conclusion:The studies demonstrated that the activities of enzymes in the medium of cultured myocardium cell could increase with the difference of coblat concentrations.The threshold value concentration of cobalt which could cause myocardium cell damage is 40 μg/ml.%目的：在体外培养大鼠心肌细胞培养液中加入不同剂量钴，观察其中心肌酶活性的改变，确定钴引起心肌细胞损害的阈浓度。方法：取新生1～3 d Wistar大鼠心室肌组织，按常规法进行细胞培养，待细胞达到亚融合状态时，向细胞培养液中加入不同剂量钴，观察心肌细胞培养液中心肌酶活性的改变。结果：钴浓度在10～20 mg/L时，对天冬氨酸转氨酶(AST)、肌酸激酶(CK)、乳酸脱氢酶(LDH)、羟丁酸脱氢酶(HBDH)活性影响不大；当钴浓度增加到40 mg/L时，AST、CK、LDH和HBDH活性有了明显升高。结论：钴浓度越高，心肌酶活性改变越大，从中选出了能引起心肌细胞损害的阈浓度。