盐霉素对人膀胱癌5637细胞的生长抑制作用

摘要

Objective To explore the influence of salinomycin in the growth, apoptosis and invasion of human bladder cancer 5637 cells,and to clarify its possible mechanism.Methods The human bladder cancer 5637 cells cultured invitro at logarithmic growth phase were divided into control group and different doses of salinomycin(15, 30 and 60μmol·L-1 )groups.The inhibitory rate of the growth of 5637 cells in various groups was measured by MTT assay. Flow cytometry was used to detect the apoptotic rates of 5637 cells in various groups. The invasiveness of 5637 cells was tested by Matrigel Invasion Assay.The expression levels ofβ-catenin protein in 5637 cells in various groups were determined by Western blotting method. Results Compared with control group,the inhibitory rates of growth of human bladder cancer 5637 cells in different doses of salinomycin groups were increased significantly(P<0.05);the apoptotic rates were increased(P<0.05).the number of cells passed the Matrigel was decreased(P<0.05),and the expression level ofβ-catenin protein was decreased (P<0.05).Compared with low dose of salinomycin group,the inhibitory rate of growth of 5637 cells in high dose of salinomycin group was increased(P<0.05);the apoptotic rate was increased(P<0.05),the number of cells passed the Matrigel was decreased (P < 0.05 ), and the expression levels of β-catenin protein was decreased (P<0.05). Conclusion Salinomycin can inhibit the growth of 5637 cells significantly,increase the apoptosis,and decrease the cell invasion;the inhibitory effect may act by inhibiting the Wnt/β-catenin pathway.%目的：探讨盐霉素对人膀胱癌5637细胞生长、凋亡和侵袭力的影响，阐明其可能的作用机制。方法：取体外培养的对数生长期人膀胱癌5637细胞，分为空白对照组和不同剂量（15、30和60μmol·L-1）盐霉素组。MTT比色法检测各组人膀胱癌5637细胞的生长抑制率，流式细胞术检测各组人膀胱癌5637细胞的细胞凋亡率，Matrigel侵袭实验检测各组人膀胱癌5637细胞的侵袭能力，Western蛋白印迹实验检测各组人膀胱癌5637细胞中 Wnt／β-catenin途径β-catenin蛋白表达水平。结果：与空白对照组比较，各剂量盐霉素组膀胱癌5637细胞的生长抑制率明显升高（P＜0.05），细胞凋亡率明显升高（P＜0.05），细胞穿膜数目减少（P＜0.05），β-catenin蛋白表达水平减少（P＜0.05）。与低剂量（15μmol·L-1）盐霉素组比较，高剂量（60μmol·L-1）盐霉组人膀胱癌5637细胞生长抑制率升高（P＜0.05），细胞凋亡率升高（P＜0.05），细胞穿膜数目减少（P＜0.05），β-catenin蛋白表达量减少（P＜0.05）。结论：盐霉素对人膀胱癌5637细胞的生长有较明显抑制作用，促进细胞凋亡，降低细胞侵袭力，并且这种抑制作用可能是通过抑制 Wnt／β-catenin信号传导途径来实现的。