人参二醇组皂苷对家兔急性酒精性心肌损伤的保护作用及其机制

摘要

Objective To observe the protective effect of Panaxadiol Saponins (PDS)on rabbit heart failure model induced by acute alcohol infusion, and to explore its action mechanism of protecting myocardium. Methods 1 5 healthy rabbits were randomly divided into control group, model group and PDS group, 5 rabbits in each group.The rabbits in control group were given 0.2 g·mL-1 saline by intravenous drip at constant speed,the rabbits in model group were given 20% ethanol with same method, and the rabbits in PDS group were given 0.025 g·kg-1 PDS by intravenous injection before intravenous drip of 20% ethanol.The hemodynamic changes were observed by ventricular intubation;the levels of serum lactate dehydrogenase (LDH),creatine kinase (CK), and creatine kinase isoenzyme (CKMB)were determined by colormetric method.The level of malondialdehyde (MDA)in myocardial tissue homogenate,the activities of superoxide dismutase (T-SOD),glutathione peroxidase (GSH-Px)and catalase (CAT)were also detected.Results Compared with control group,the left ventricular end-diastolic pressure (LVEDP)of the rabbits in model group was significantly decreased at 30 min(P<0.05);the serum LDH,CK and CKMB levels were increased(P<0.05),the MDA level in myocardial tissue homogenate was increased(P<0.05),and the T-SOD,GSH-Px and CAT activities were decreased (P<0.05).Compared with model group,the LVEDP of the rabbits in PDS group was increased,the serum LDH,CK,and CKMB levels were decreased(P<0.05),the MDA level was decreased(P<0.05),and the activities of T-SOD,GSH-Px and CAT were increased(P<0.05).Conclusion PDS has protective effect on heart failure induced by acute alcohol infusion,and its mechanism may be related to the improvement of cardiac peroxidation.%目的：探讨人参二醇组皂苷（PDS）对家兔酒精性心力衰竭的保护作用，阐明 PDS保护心肌的作用机制。方法：15只健康家兔随机分为对照组、模型组和 PDS 组，每组5只。对照组家兔静脉滴注生理盐水0.2 g·mL-1，模型组家兔恒速静脉滴注20％酒精（0.1 g·kg-1·min-1），PDS 组家兔静脉滴注酒精前给予PDS 0.025 g·kg-1。心室内插管检测各组家兔的血流动力学变化；比色法检测家兔血清中乳酸脱氢酶（LDH）、肌酸激酶（CK）和肌酸激酶同工酶（CKMB）水平，检测心肌组织匀浆中丙二醛（MDA）水平、总超氧化物歧化酶（T-SOD）、谷胱甘肽过氧化物酶（GSH-Px）和过氧化氢酶（CAT）活性。结果：与对照组比较，模型组家兔左心室内舒张末期压（LVEDP）在开始记录30 min时显著降低（P＜0.05），血清 LDH、CK 和 CKMB水平升高（P＜0.05），心肌组织匀浆中 MDA水平升高（P＜0.05），T-SOD、GSH-Px和 CAT活性下降（P＜0.05）；与模型组比较，PDS组家兔 LVEDP显著升高，血清 LDH、CK及CKMB水平下降（P＜0.05），心肌组织匀浆中 MDA水平下降（P＜0.05），T-SOD、GSH-Px及CAT活性升高（P＜0.05）。结论：PDS对家兔急性酒精性心力衰竭具有保护作用，其作用机制可能与改善心肌过氧化损伤有关。