人参黄连组合物对糖尿病肾病小鼠的治疗作用

摘要

目的：探讨人参黄连组合物对糖尿病肾病（DN）小鼠生化指标和肾脏病理组织形态的作用，为其应用于DN治疗提供依据。方法：取10只C57小鼠设为正常对照组，另取240只C57小鼠采用高糖高脂饲料联合小剂量链脲菌素（STZ）诱导制备 DN 模型，将成模的小鼠随机分为模型对照组和低（80 mg · kg－1＋20 mg·kg－1）、中（160 mg·kg－1＋40 mg·kg－1）、高（320 mg·kg－1＋80 mg·kg－1）剂量人参黄连组合物组及黄连总生物碱组（320 mg·kg－1）、人参总皂苷组（80 mg·kg－1）、卡托普利（8 mg·kg－1）组，每组10只。于用药8周后观察各组小鼠的一般生理状况，检测各组小鼠空腹血糖（FBG）、尿微量白蛋白（MAU）、血肌酐（Scr）、血清尿素氮（BUN）、总胆固醇（TC）和甘油三酯（TG）水平和24小时尿量；HE染色观察小鼠肾脏病理组织形态表现。结果：与模型对照组比较，各剂量人参黄连组合物组小鼠尿量和体质量增加等一般情况明显改善， FBG水平降低（P＜0.05）；MAU及血清 BUN、TC和 TG水平降低（P＜0.05）。各剂量人参黄连组DN小鼠肾小球-肾间质病变明显改善。结论：人参黄连组合物对早期DN具有一定治疗作用。%Objective:To explore the effect of Ginseng and Coptis composition on the biochemical indexes and morphology of kidney tissue in the diabetes nephrophthy (DN)mice,and to provide basis for its application in DN treatment.Methods:Ten C57 mice were selected as normal control group.A total of 240 C57 mice were induced to establish DN models by feeding with high-sugar and high-fat diet combined with small dose of streptozotocin (STZ).The DN mice were randomly divided into model control group,low (80 mg· kg-1+20 mg· kg-1 ), medium (160 mg·kg-1+40 mg·kg-1 ),and high (320 mg·kg-1+80 mg·kg-1 )doages of Ginseng and Coptis composition groups,total alkaloid from Coptis group (320 mg·kg-1 ),total Ginsenoside group (80 mg·kg-1 ), and captopril group (8 mg·kg-1 );there were 10 mice in each group.After treated for 8 weeks,the physiological status of C57 mice in various groups were observed.The levels of fasting blood glucose (FBG),microalbuminuria (MAU),serum creatinine (Scr),blood urea nitrogen (BUN),total cholesterol (TC),and triglyceride (TG)of mice in various groups were measured. The pathomorphology of kidney tissue of mice was observed by HE staining.Results:Compared with model control group,the physiological status of C57 mice in different dosages of Ginseng and Coptis composition groups, such as the urine volume and the body weight increase were significantly improved,and the levels of FBG were decreased (P<0.05);the levels of MAU,BUN,TC,and TG were also decreased (P<0.05).The glomerular and renal interstitial lesions of the kidney tissue in different dosages of Giseng and Coptis composition groups were significantly improved. Conclusion:Ginseng and Coptis composition has therapeutical effect on early DN.