“温阳补气”针法对EAMG大鼠神经肌肉接头处AchR蛋白表达的影响

摘要

Objective:To study the influence of “Warming Yang and Nourishing Qi”Acupuncture in the expression levels of acetylcholine receptor protein (AchR)in gastrocnemius neuromuscular junction (NMJ)of the rats with experimental autoimmune myasthenia gravis (EAMG),and to clarify the mechanism of “Warming Yang and Nourishing Qi”Acupucture in the treatment myasthenia gravis (MG).Methods:The female Lewis rats were used to establish the EAMG rat models immuned with AchRα1 129-145 polypeptide fragments.30 EAMG rats were selected randomly and divided into model control group, drug control group, and acupuncture treatment group (n=10),and another 10 rats purchased at the same period were used as blank control group.The rats in drug control group were treated with 1 8.5 mg · kg-1 · d-1 pyridostigmine by gavage. And the rats in acupuncture treatment group were treated with irritating by“Warming Yang and Nourishing Qi”Acupuncture.The rats in blank control group and model control group were not treated .The Lennon symptom scores of the rats in various groups were recorded.After treatment,the tissues of gastrocnemius NMJ of the rats were taken,and the AchR protein expression levels in NMJ of the rats in various groups were detected by Western blotting method. Results:Compared with blank control group,after successfully modelling,the Lennon symptom scores of the rats in model control group, drug control group and acupuncture treatment group before treatment were significantly increased (P<0.01).Compared with model control group,the Lennon symptom scores of the rats in drug control group and acupuncture treatment group after two treatment course were significantly increased (P<0.01 ). Compared with blank control group, the AchR protein expression level in gastronemus NMJ of the rats in model control group was significantly decreased (P<0.01). Compared with model control group, the AchR protein expression levels in gastronemus NMJ of the rats in acupuncture treatment group and drug control group after treatment were significantly increased (P<0.01).Conclusion:“Warming Yang and Nourishing Qi”Acupuncture has good therapeutic effect on the EAMG through increasing the NMJ AchR protein expression level of the EAMG rats.%目的：探讨“温阳补气”针法对实验性自身免疫性重症肌无力（EAMG）大鼠腓肠肌神经肌肉接头（NMJ）处乙酰胆碱受体（AchR）蛋白表达水平的影响，阐明针灸治疗重症肌无力（MG）的作用机制。方法：采用AchRα1129-145多肽片段免疫接种 Lewis雌性大鼠，建立 EAMG大鼠模型。在建模成功的 EAMG大鼠中随机选取30只，分为模型对照组10只、药物对照组10只和针刺治疗组10只，并将同期购进的未建模的10只大鼠设为空白对照组。药物对照组大鼠予以18.5 mg· kg－1· d－1溴吡斯的明灌胃治疗；针刺治疗组大鼠予以“温阳补气”针法治疗；其余2组不予任何处置作为对照。记录治疗前后各组大鼠 Lennon症状评分。治疗结束后，取4组大鼠腓肠肌 NMJ 处组织，采用 Western blotting 法检测各组大鼠 NMJ 处 AchR蛋白表达水平。结果：与空白对照组比较，建模成功后治疗前模型对照组、药物对照组和针刺治疗组大鼠 Lennon症状评分明显升高（P＜0.01）；与模型对照组比较，经过2个疗程治疗后药物对照组和针刺治疗组大鼠 Lennon症状评分均明显降低（P＜0.01）；与空白对照组比较，模型对照组大鼠腓肠肌 NMJ 处 AchR 蛋白表达水平明显降低（P＜0.01）；与模型对照组比较，治疗后针刺治疗组和药物对照组 EAMG大鼠腓肠肌 NMJ 处 AchR蛋白表达水平明显升高（P＜0.01）。结论：“温阳补气”针法可以提高 EAMG 大鼠 NMJ 处 AchR 蛋白表达水平，发挥治疗EAMG的作用。