高效液相色谱-电感耦合等离子体质谱法测定化妆品中2-溴-2-硝基-1,3-丙二醇

摘要

建立了高效液相色谱-电感耦合等离子体质谱(HPLC-ICP-MS)测定化妆品中2-溴-2-硝基-l,3-丙二醇的方法.采用ZORBAX RX-C8色谱柱(150 ×2.1 mm,5 μm)进行分离,以甲醇-水-5％磷酸(10∶985∶5,用2 mol/L NaOH调至pH 3.0)为流动相,流速为0.7 mL/min,柱温为室温.结果显示,2-溴-2-硝基-1,3-丙二醇在2.5 min处出峰,其线性范围为0.01々 1.0 mg/L,相关系数为0.999 9.不同基质化妆品的方法检出限均为1.0 μg/g,回收率为94.6％ ～ 101.3％,相对标准偏差(RSD,n=6)为1.5％ ～4.5％.该方法快速、准确、灵敏、无干扰,适用于化妆品中2-溴-2-硝基-1,3-丙二醇的定性定量测定.%A new method was established to determine 2-bromo-2-nitro-1,3-propanediol in cosmetics by high performance liquid chromatography-inductively coupled plasma mass spectrometry(HPLC-ICP-MS).The analyte was separated on a ZORBAX-C8 column (150 ×2.1 mm,5 μm) using methanol-water-5％ phosphoric acid (10 ∶ 985 ∶ 5,pH value was adjusted to 3.0 with 2 mol/L NaOH) as mobile phase.The flow rate was set at 0.7 mL/min.The column temperature was room temperature.The results indicated that the calibration curve of 2-bromo-2-nitro-1,3-propanediol was linear in the range of 0.01-1.0 mg/L,with a correlation coefficient(r) of 0.999 9.The detection limits of different matrix samples were all 1.0 μg/g.The spiked recoveries of 2-bromo-2-nitro-1,3-propanediol ranged from 94.6％ to 101.3％,with RSDs (n =6) of 1.5％-4.5％.The method showed the advantages of rapidness,sensitivity,accuracy and little interference,and it could be used for the qualitative and quantitative determination of 2-bromo-2-nitro-1,3-propanediol in cosmetics.