糙叶五加根皮化学成分研究

摘要

目的 基于抗炎活性研究糙叶五加根皮的化学成分.方法 采用XAD-4、正相硅胶、ODS、Sephadex LH-20等常规柱色谱技术结合实时直接分析质谱技术(DART-MS)进行分离纯化,并根据其理化性质和波谱数据鉴定结构.结果 通过常规柱色谱从糙叶五加根皮中分离鉴定了7个化合物,分别为:豆甾醇(1)、β-谷甾醇(2)、海松酸(3)、(+)-芝麻素(4)、洒维宁(5)、丁香苷(6)和刺五加苷E(7)；另外,通过实时直接分析质谱与标准图谱比较推测糙叶五加根皮中可能存在taiwanin C、10-羟基-2,8-癸二烯-4,6-二炔酸、3,7,11-trimethyl-2,6,10-dodecatrienoic acid、咖啡酸和stigmast-7-ene-3,6-diol.结论 化合物3,4,5,7为首次从该种植物中分离得到.%Objective To study the chemical constituents of root barks of Acanthopanax henryi (A. Henryi) based on anti-inflammatory activities. Methods The compounds were isolated and obtained by column chromatography including XAD -4, silica gel, ODS and Sephadex LH -20, combined with DART -MS technology. The structures of these compounds were elucidated by means of physiochemical properties and spectroscopic analysis. Results Seven compounds were separated and identified as stigmasterol(1), β-sitosterol(2), pimaric acid(3), (+)-sesamin(4), savinin (5), syringing (6) and eleutheroside E (7) by column chromatography. In addition, taiwanin C, 10-hydroxy-2,8-decadiene-4,6-diynoic acid, 3,7,11-trimethyl-2,6,10-dodecatrienoic acid, caffeic acid together with stigmast-7-ene-3,6-diol could be existed in root barks of Acanthopanax henryi by DART-MS compared with their standard spectrums. Conclusion Compounds 3, 4, 5, 7 were obtained from the genus for the first time.