斑马鱼胚胎急性毒性实验法检测赶山鞭化学成分胚胎发育毒副作用

摘要

Objective To detect the toxic and side effects of chemical components from Hypericum attenuatum on embry-onic development of zebrafish by acute toxicity test. Methods The 6 h post-fertilization zebrafish was used as experimen-tal models, randomly divided into the control group with DMSO and the sample groups with quercetin, rutin, hyperin, quercertin -3 -O -α -L -arabino furanoside, quercertin -3 -O -β -D -glucoside of Hypericum attenuatum. The mortality, malformation, hatchability at 72 hpf (72 hours after fertilization) were observed. Results The five constituents show obvious inhibiting hatchability on embryonic development of zebrafish (P<0.05), which show stronger inhibition at high concentra-tion, with dose-dependent effect. Conclusion The five components may have a certain toxicity to embryonic development.%目的 利用斑马鱼胚胎急性毒性实验检测赶山鞭化学成分胚胎发育毒副作用.方法 以孵育6 h的斑马鱼胚胎为实验模型,设DMSO对照组和quercetin,rutin,hyperin,quercertin-3-O-α-L-arabinofuranoside,quercertin-3-O-β-D-glu-coside五种赶山鞭单体化合物样品组,观察记录72 hpf(受精后72小时)时的致死率、致畸率、孵化率.结果 72 hpf,在各浓度梯度范围内,5种化合物对斑马鱼胚胎致死致畸抑制孵化效应明显(P<0.05),高浓度时愈加明显(P<0.01),且呈现一定的剂量依赖效应.畸形类型主要为心房囊水肿、体节弯曲.结论 上述5种化合物可能对胚胎发育有一定的毒性.