基于液滴微流控芯片的单细胞分离

摘要

We developed a droplet-based single cell isolation microfluidic device to collect the single cell droplets for the further single cell assays.Based on the fabrication technique of microfluidics, we designed and fabricated the PDMS chip with flow-focusing structure.We used the flow-focusing structure microfluidic device to generate alginate hydrogel droplets which contain single cell by changing flow rate of the dispersed phase and continuous phase.Then we statistically analyzed the number of encapsulated cells in these droplets.And the number of single cell droplets was less than 10% of the number of total droplets.By integrating microelectrode into the microfluidic chip and inspired by the self-charged phenomenon of the aqueous droplet, we achieved the single cell droplet isolation and enrichment using electrostatic force which was generated by the pulse of voltage in the microelectrode.%开发基于静电场力和集成微电极的微液滴操控技术以实现单细胞的分离.结合微流控技术的发展,设计并制作了以有机聚合物PDMS( polydinethylsiloxane)为材料的具有流聚焦结构的芯片,利用流聚焦结构的芯片,通过改变分散相和连续相的流速,制备包裹着悬浮癌细胞( HCT116)的海藻酸钠凝胶溶液微液滴,并对其包裹细胞数目进行统计分析,其中包裹单细胞的微液滴少于总液滴数的10％.受到液滴自带电现象的启发,通过集成微电极到微流控芯片中,利用电压脉冲产生静电场力,实现了单颗单细胞微液滴的电分离和富集.