Conditioned Medium from Human Umbilical Vein Endothelial Cells Promotes Proliferation,Migration,Invasion and Angiogenesis of Adipose Derived Stem Cells

摘要

Preeclampsia(PE) is a pregnancy-specific hypertensive complication,closely related to endothelial dysfunction.Adipose derived stem cells(ADSCs) have the capacity to differentiate into endothelial cells for vascular repair.Therefore,we hypothesized that induced endothelial differentiation of ADSCs might hold great potential for the treatment of PE.In this study,the primary ADSCs and human umbilical vein endothelial cells(HUVECs) were isolated by the collagenase digestion method.The supernatant of HUVECs was collected from the first generation of cells.Then,ADSCs were divided into two groups:ADSCs alone group and induced ADSCs(i ADSCs) group.In i ADSCs group,ADSCs were induced by HUVECs conditioned medium and ADSCs special culture medium at a ratio of 1:1 over a two-week period.In order to identify the endothelial characteristics of i ADSCs,CD31 and CD34 were examined by flow cytometry.The proliferation,migration,invasion and angiogenesis assays were employed to compare the bioactivity of i ADSCs and ADSCs.Furthermore,The levels of angiogenic related factors including vascular endothelial growth factor(VEGF) and placenta growth factor(Pl GF) were detected by RT-PCR and Western blotting.Results showed conditioned medium from HUVECs promoted ADSCs proliferation,migration,invasion and angiogenesis.In addition,the levels of VEGF and Pl GF were significantly enhanced in i ADSCs group.This study uncovered the i ADSCs application potential in the therapy and intervention of PE.