玉米叶高点长等叶型性状QTL定位及上位性效应分析

摘要

以由综3×豫87-1衍生的一套包含223个家系的RIL群体为材料,通过3个地点的表型鉴定,借助由1243个SNP标记构建的遗传连锁图谱对玉米叶高点长、叶长、叶宽和叶面积进行QTL定位及上位性效应分析.结果表明,4个叶型性状共检测到10个显著的QTL,这些QTL与环境的互作均未达到显著水平.在这些QTL中,调控叶高点长的qLf5-1,qLf8-1和调控叶长的qLL8-1,分别解释表型变异的7.20％,6.06％和6.39％,说明这3个主效QTL是调控叶型性状的重要位点.上位性效应分析共检测到6对位点间互作,互作效应为2.30％～7.39％,属于非显著QTL位点对互作,其中2对位点互作的上位性效应与环境互作效应显著,说明上位性互作效应在叶型性状的遗传中占有一定的比例,同时也受环境的影响.%Using a set of RIL populations containing 223 pedigrees derived from Zong 3 × Yu 87-1 as material,the phenotypes of the three sites were used to identify the height of the maize leaves using genetic linkage maps constructed from 1243 SNP markers for leaf length,leaf width and leaf area for QTL mapping and epistasis analysis.The results showed that 10 significant QTLs were detected in 4 leaf-type traits.The interactions between these QTLs and the environment did not reach significant levels.In these QTLs,qLf5-1,qLf8-1,and qLL8-1,which regulate long leaf height,accounted respectively for 7.20％,6.06％,and 6.39％ of phenotypic variation,indicating that these three major QTLs are regulating the important locus of leaf-shaped traits.By epistasis effect analysis,a total of 6 pairs of sites interactions were detected,and the interaction effect was 2.30％～7.39％,which belonged to non-significant QTL site interaction,in which the epistasis effect and environment interaction of the two pairs of sites interacted with each other.The effect was significant,indicating that epistatic effects accounted for a certain proportion of the inheritance of leaf-type traits,but also affected by the environment.