苯磺隆对鲫鱼肝细胞内膜系统形态和转氨酶活性的影响

摘要

为揭示苯磺隆对水生生物细胞生物标志物——内膜系统和转氨酶[谷丙转氨酶(GPT)、谷草转氨酶(GOT)]的影响,采用等比数列设计质量浓度梯度对鲫鱼进行慢性中毒试验.结果表明:随苯磺隆质量浓度的增加,鲫鱼肝细胞内膜系统中线粒体肿胀及空泡化,内质网空泡化,异噬型溶酶体增多.0.64～5.07 mg/L的苯磺隆处理24 h、48 h、96 h,均使上述2种转氨酶活性增强,96 h、10.14 mg/L苯磺隆处理组GPT活性明显低于同等质量浓度下48 h处理组,提示苯磺隆对水生生物标志物具有一定的毒性影响.%To study the effects of tribenuron-methyl on biomarkers of aquatic organism cells in the intima and on two aminotransferase, GPT and GOT, a chronic poisoning test was performed by geometric sequence design concentration gradient and the experiment data was analyzed by factorial design variance. The results showed that with concentration elevated, mitochondrion swelled and vacuolated,ER vacuolated,heterolysosome grew in the hepatic endomembrane system. Treatment of tribenuron fluid lower of 0. 64 — 5. 07 mg/L caused the enhancement of two transaminase activity. However,the group of high concentration of 10. 14 mg/L decreased their activities after 96 h,indicating that tribenuron-methyl has toxicity impact on aquatic biomarkers.