Fmoc is usually used as an amino-protecting group.The traditional removal method is realized by using a secondary amine.However,this method has some shortcomings including high cost,difficult recycle of excess secondary amine and envi-ronmental pollution.The paper reports a novel heterogeneous deprotection method of Fmoc group using a new inorganic base, namely,nano-potassium carbonate.Nano-potassium carbonate with average particle size 64 nm is prepared using wet grinding method.The deprotection of Fmoc-Phe-OBn is preformed as a Model reaction.The optimum conditions are that reaction solvent is absolute ethanol,molar ratio of Fmoc-L-Phe-OBn to nano-K2CO3is 1 ︰ 2,and reaction temperature is 25 ℃.The corre-sponding free amino compound is obtained with high yield.To further explore the scope and limitations of this method,seven substrates including Fmoc-anmino acids and dipeptides are examined.All the yields are above 98%.The nano-K2CO3could be regenarated and reused for 5 times without loss of its reactive activity.This method has the advantages of simple operation,low cost,small pollution and recyclable potassium carbonate,and has important reference value for the research on the synthesis and deprotection of polypeptides.%Fmoc是一种常用的氨基保护基,传统的脱除方法是通过加入二级胺与之作用来实现.针对方法中存在的成本较高、使用的过量二级胺不易回收以及污染环境等缺点,建立了利用新型无机碱纳米碳酸钾非均相脱除Fmoc保护基的新方法.采用湿法研磨方法,将普通碳酸钾制成粒径为64 nm的纳米碳酸钾,以Fmoc保护的 L-苯丙氨酸的脱保护反应为 Model 反应,确定了最佳反应条件:反应溶剂为无水乙醇,Fmoc-L-Phe-OBn 与纳米碳酸钾物质的量比为 1︰2,反应温度为 25℃,高产率得到了相应的游离氨基化合物,进一步研究了新方法的适用范围,对 7 个 Fmoc 保护的氨基酸和二肽的底物进行了测试.结果表明,产率均在 98%以上,且纳米碳酸钾能够回收再利用,在 5 次之内的活性几乎没有变化.新方法具有操作简单、成本低、污染小、碳酸钾可回收利用的优点,对多肽的合成及脱保护研究有重要的参考价值.
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