三参方对慢性萎缩性胃炎大鼠氧自由基及胃黏膜组织形态的影响

摘要

Objective: to explore the functional mechanism of Sanshen Fang in treating chronic atrophic gastri-tis.Methods: The rats were randomly divided into control group , model group, Weimeisu group, Sanjiu Weitai group, high-dose of Sanshen Fang group and low-dose group, models made by composite factors.The experiment lasting for 8 weeks was to detect the changes of serum SOD activity and MDA content , and to observe the gastric mucous membrane tissue change.Results: Compared with control group, MDA content of model group increased significantly, SOD activity decreased significantly ( P <0.01); Epithelial cells of gastric mucous membrane had degeneration, necrosis and abscission, glands saw atrophy to different degrees , and there were a great deal of in-flammatory cell infiltration.Compared with model group, MDA of treatment groups decreased and SOD increased ( P <0.01 or P <0.05); Morphology indicated that gastric mucous membrane tissues were improved greatly .Conclusion: Sanshen Fang can reinforce SOD activity, decrease MDA content, clear oxygen radicals, has the ac-tion of antioxidation, and can improve gastric mucous membrane pathological appearance .%　　目的：探讨三参方治疗慢性萎缩性胃炎的作用机制。方法：将实验大鼠随机分为正常组、模型组、维霉素组、三九胃泰组和三参方高、低剂量组，采用复合因素造模法复制大鼠慢性萎缩性胃炎模型，实验8周后，测定各组大鼠血清 SOD 活性和 MDA 的含量变化，并观察胃黏膜组织形态变化。结果：与正常组比较，模型组大鼠血清 MDA 含量明显升高、 SOD 活性明显降低（ P ＜0.01）。胃黏膜上皮细胞变性、坏死、脱落，腺体有不同程度的萎缩，间质内有大量炎性细胞浸润。与模型组比较，各治疗组大鼠血清 MDA 含量降低、 SOD 活性升高（ P＜0.01或 P ＜0.05），形态学结果显示胃黏膜组织形态有明显改善。结论：三参方能增强 SOD 活性，降低 MDA 含量，有效清除氧自由基，具有良好的抗氧化作用；并对胃黏膜的病理形态改变也有较好的改善作用。